Tissue Culture


 
 
Concept Explanation
 

Tissue Culture

Tissue culture involves maintaining of tissues or organs (in case of plant tissue culture isolated cells or small pieces of plant tissues are used) under sterile or  in vitro conditions in nutrient cultures to grow into new disease-free plants. This technique is also known as in vitro micropropagation because it takes place in a laboratory and the plants are transferred from the labs to the soil.

Plant tissue culture is widely used for the rapid multiplication of plant species with superior qualities. This method involves following steps:

  • In this method, small pieces are cut from the plant like flower buds, stem tissue, growing tips, leaves, etc., (known as ex-plants).
  • These a then transferred to an artificial medium where they divide rapidly to form a callus (undifferentiated mass of cells).
  • This callus is then transferred to fresh medium containing hormones for growth and differentiation, where it subsequently develops into platelets.After this, the developed platelets are placed in the soil to grow into a mature plant.
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    Sample Questions
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    Question : 1

    Plant that are derived of a single from the vegetative organs of a single plant are known as _________________

    Right Option : A
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    Question : 2

    Some plants like _______________________ can be grown by the method of tissue culture.

    Right Option : D
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    Explanation
    Question : 3

    Carbohydrate extracted from red algae which is used as a medium in tissue culture is ___________________

    Right Option : B
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    Explanation
     
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